service - Cryo-EM SPA

B A C K

Cryo-EM Services for High Resolution Protein Structure Determination

In the past few years, single-particle cryo-EM in particular has triggered a revolution in structural biology and has become a newly dominant discipline.Today,Single Particle Analysis(SPA)is no longer a complementary technique but a dominant one, changing the field of structural biology in a profound and unprecedented way and facilitating major new discoveries.

Cryo-EM Services for High Resolution Protein Structure Determination

Cryo-EM SPA Workflow

Cryo-EM SPA Workflow

Why do you need Cryo-EM SPA Services?

● small amount
● a large biomolecular complex
● hard to crystallize
More far-reaching significance:
● Pursuing highly innovative protein structure analysis
● Revealing complex conformational mechanisms in the process of protein function
● Advance the design and optimization of next-generation inhibitors, regulators and other related ligands

Why choose ShuimuBio for Cryo-EM SPA?

ShuimuBio hosts two high-end cryo-electron microscopes and established powerful, stable, and versatile system for delivering high-resolution 3D structure of biological samples in their near-native state. Our cryo-EM services are designed to support a full range of project types from data acquisition to project-based proposal. We are able to complete a structure determination project within two to three weeks for well-behaved samples.

About the samples

Protein (50-100 ul,≥2mg/mL,Purity >95%):

SDS-PAGE without obvious stray bands and degradation bands, also to ensure that it is the target protein (mass spectrometry identification, etc.).

Buffer (50-100 ml):

Do not contain organic substances such as polysaccharides, DMSO, glycerol (otherwise it will affect the lining), salt ion concentration below 300 mM.

Homogeneity:

Before negative staining, try to do gel filtration chromatography on the sample (this step is necessary if it is a complex), if the result shows a single peak, it proves that the sample homogeneity is greater than 90%. (Glycosylation or phosphorylation modifications have less effect on the electron microscopic structure resolution); after gel filtration layer, do not concentrate again to avoid protein aggregation, etc.

Storage (10ul-20ul/tube):

It is recommended that samples be dispensed directly after preparation to avoid the need for repeated freezing and thawing during the experiment, resulting in unusable samples.

Contact.

  • E-mail: Hi@shuimubio.com
  • Contact a Specialist: +86 185 1180 1010

Address | ShuimuBio HQ

F4, Bio² Innovation Center, Life Science Park,
Changping District, Beijing, PR China

Address | ShuimuBio CryoEM Center

B1, National Institute of Biological Sciences,
Changping District, Beijing, PR China

  • E-mail: Hi@shuimubio.com
  • Contact a Specialist: +86 185 1180 1010

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